Fig 1: Histological and immunohistochemical evaluation of recellularized vascular grafts. Histological evaluation of the seeded, statically cultivated and immediately examined vascular graft (rTEVG-s) with H&E staining revealed multiple, morphologically round cells discontinuously attached to the basal membrane of the vascular graft (A). Further immunohistochemical staining with important endothelial cell markers such as CD31 (B), CD34 (C), eNOS (D) and vWF (E) revealed the majority of the cells were of endothelial phenotype. A few cells also stained positive for CD90, indicating a much lower number of hMSC dispersed within the hEPC (F). Histological evaluation of the dynamically cultivated vascular grafts (rTEVG-10d) displayed morphologically elongated cells due to introduction of gradually increasing medium flow and shear stress (G). Further characterization with CD31 (H), CD34 (I), eNOS (J) and vWF (K) revealed that while the cells stained strongly for eNOS, CD31 and vWF, staining with CD34, a marker of immature EPC was much weaker, a finding which was interpreted to point to cell maturation. Moreover, staining with CD90 antibody revealed scarce hMSC dispersed and attached to the basal membrane (L). Lastly, histological characterization of the vascular grafts perfused over 14 days (rTEVG-14d) revealed morphologically irregular cells (M), which stained slightly positive for CD31 (N) and vWF (O) and did not stain for CD34 (P), eNOS (Q) and CD90 (R), findings in line with the cell culture parameters, that pointed to excessive cell detachment and death after the 10th day of cultivation. Scale bar represents 50 µm
Fig 2: Overexpression of PIK3CA promotes sunitinib resistance in RCC. (A) Subcutaneous xenograft growth in nude mice under different treatment conditions (upper panel). Anatomical picture of subcutaneous xenografts in nude mice (lower left panel) and growth curve of subcutaneous xenografts (lower right panel). (B) Representative immunohistochemical results of PIK3CA, CD31 and CD34 in xenografts. Scale bar, 100 µm. Results are presented as the mean ± SD. *P<0.05. PIK3CA, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; RCC, renal cell carcinoma.
Fig 3: Overexpression of QPCT promotes tumor angiogenesis. (A) Representative immunohistochemical results of QPCT, CD31 and CD34 in xenografts. Scale bar, 100 µm. (B) Representative results of HUVEC tube formation cultured with the supernatant of RCC cells overexpressing QPCT. (C) Representative results of HUVEC tube formation cultured with purified QPCT cytokines (rhQPCT). The group incubated with exogenous VEGF was used as the positive control group. Total tube length was calculated using ImageJ software. Results are presented as the mean ± SD. *P<0.05, **P<0.01. QPCT, glutaminyl peptide cyclotransferase; CM, conditioned medium.
Fig 4: Effects of adiponectin depletion on muscle stem cell regeneration in the soleus muscles and gastrocnemius at 2 months post-ET. (A–C) Representative images and quantitative data for muscle stem cell regeneration identified by double immunofluorescent staining with rabbit mAb against CD34 (green) and goat pAb against integrin-a7 (red). Arrowheads: related positive-staining cells. Data are mean ± SEM (n = 8–9). (D,E) Fluorescence staining of muscles with laminin 5 rabbit pAb (green) and desmin mAb (red). Scale bar: 50 µm.
Fig 5: Histological and immunohistochemical evaluation of seeded vascular grafts after perfusion with whole blood. Histological evaluation of the whole blood perfused seeded grafts revealed findings similar to the seeded, non-blood perfused grafts, whereby cells were attached to the basal membrane (A). Staining with anti-CD31 (B), anti-CD34 (C), anti-eNOS (D) and anti-vWF antibody (E) revealed the majority of the cells were endothelial cells, while staining with anti-CD90 antibody (F) revealed the location of the hMSC. Scale bar represents 50 µm
Supplier Page from Abcam for Anti-CD34 antibody [EPR2999]